Assassin's Creed: Brotherhood

Following the events of Assassin's Creed II, Desmond Miles (Nolan North), Lucy Stillman (Kristen Bell), Rebecca Crane (Eliza Schneider), and Shaun Hastings (Danny Wallace) escape from the Templars who attacked their hideout and establish a new base in the ruins of the Villa Auditore in Monteriggioni. After restoring the electricity in the tunnels under the villa, Desmond uses the Animus 2.0 to continue reliving the genetic memories of his ancestor Ezio Auditore (Roger Craig Smith). His mission is to find the Apple of Eden, which could prevent an impending disaster coming that same year, believed to be perpetrated by the Templars.
Ezio's story continues in December 1499, as he exits the Vault and escapes Rome with his uncle, Mario (Fred Tatasciore). Returning to Monteriggioni, Ezio explains to his fellow Assassins what he saw inside the Vault and is comforted by the prospect that his personal vendetta is over; however, Niccolò Machiavelli (Shawn Baichoo) challenges Ezio's decision to leave Rodrigo Borgia (Manuel Tadros) alive. The following day, Monteriggioni is besieged by the Papal Army, commanded by Cesare Borgia (Andreas Apergis), Rodrigo's son. Cesare kills Mario and claims the Apple for the Templars. An injured Ezio escapes and travels to Rome, the center of Templar power in Italy, seeking vengeance against the Borgias. There, he discovers that the Assassin Order has lost much of its manpower and strength. Determined to rebuild the Guild, Ezio convinces Machiavelli that he can lead it, recruiting people to the Brotherhood and restoring it to its former strength.
Over the next three years, Ezio cripples the Borgias' hold in the capital, sabotaging Cesare's resources and assassinating key people close to him, while slowly restoring Rome to its former glory. After returning the Assassins to full strength, Ezio is given the rank of Il Mentore (Italian for "The Mentor") and made the de facto leader of the Assassins in Italy. During this time, Ezio's sister Claudia (Angela Galuppo) is also made an Assassin.
After learning of Ezio's actions, Cesare confronts his father to ask for more money and the Apple. Rodrigo refuses, cautious of provoking the Assassins, and attempts to kill his son with a poisoned apple, fearing Cesare's lust for power. Cesare, having only taken a bite, spits it out and forces the rest of the apple into his father's mouth, killing Rodrigo. After learning of the Apple's location, Ezio retrieves it from within St. Peter's Basilica, before using it to overwhelm Cesare's forces and withdraw the backing of his supporters. Cesare is arrested by Pope Julius II's Papal Army after Ezio and the Assassins confront him and kill his remaining followers.
A few years later, Ezio uses the Apple once more to check on Cesare, discovering he has broken out of prison. After hiding the Apple in a First Civilization Temple built underneath the Roman Colosseum, Ezio leaves Italy in pursuit of Cesare. In 1507, Cesare, with the support of his new patron, John III of Navarre, lays siege to the city of Viana in Spain. Ezio encounters Cesare on the battlements of a crumbling castle and fights him. Cesare claims that he cannot be killed by the hand of a mortal man, thus inciting Ezio to "leave [him] in the hands of fate" by dropping him off the battlements to his death.
Using the coordinates from Ezio's memories, Desmond, Lucy, Shaun, and Rebecca travel to the Temple where Ezio hid the Apple, intending to use it to locate other Temples and keep the Pieces of Eden they contain out of the Templars' reach. As Desmond enters the Temple, he is confronted with holographic apparitions of a woman called Juno (Nadia Verrucci), similar to Minerva. She comments on humanity's lack of knowledge, calling it "innocent and ignorant", before suddenly becoming angry and yelling, "We should have left you as you were!" As Desmond approaches the Apple and touches it, time freezes around him. Juno states that Desmond is descended from her race, and is their enemy; she also says there is a woman who would accompany him through "the gate," but must not be allowed to. She takes control of Desmond's body and forces him to stab Lucy. Both fall to the ground, with Lucy dead and Desmond entering a coma. As the credits roll, two men are heard discussing whether to put Desmond back in the Animus.